| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 1 | Clostridium septicum | GCAGATGGCGATGATGTTCC | ACATCATCACTTGCTGGTGC | 59.41 | 58.83 | 284 | 158 |
100.00%
|
100.00%
|
| Primer 2 | Clostridium septicum | AGCGGTAAAGGTGGAGTTGG | ACTCCAGTAGCCATTGGACC | 59.96 | 59.09 | 287 | 152 |
100.00%
|
100.00%
|
| Primer 3 | Clostridium septicum | ATTGTCCAGGGCAAGGTGG | CCAGCTCCTTGACTAGCTCC | 59.92 | 59.54 | 214 | 152 |
100.00%
|
100.00%
|
| Primer 4 | Clostridium septicum | TGCACCGGAAGATTTGACAG | GCTCTTTGCCTTTGACCACC | 58.48 | 59.68 | 183 | 151 |
100.00%
|
100.00%
|
| Primer 5 | Clostridium septicum | AGCGGTAAAGGTGGAGTTGG | CAGTAGCCATTGGACCTCTCC | 59.96 | 59.86 | 283 | 142 |
100.00%
|
100.00%
|
| Primer 6 | Clostridium septicum | TGATGCAACTTGCCCTATGG | TGAGTAGGACAGAAGCCTTGG | 58.23 | 59.10 | 279 | 139 |
100.00%
|
100.00%
|
| Primer 7 | Clostridium septicum | ATGATGCAACTTGCCCTATGG | TGAGTAGGACAGAAGCCTTGG | 58.69 | 59.10 | 280 | 139 |
100.00%
|
100.00%
|
| Primer 8 | Clostridium septicum | TGGGCAAACAAAATTTGGTGC | TGTTGTTGCTGCAAGTCTTCC | 58.98 | 59.59 | 151 | 138 |
100.00%
|
100.00%
|
| Primer 9 | Clostridium septicum | GCCCTTGGTGTTCGAATCC | GCACTTTGTGGGCATTCATTC | 58.82 | 58.66 | 208 | 133 |
100.00%
|
100.00%
|
| Primer 10 | Clostridium septicum | TGCCCTTGGTGTTCGAATCC | GCACTTTGTGGGCATTCATTC | 60.61 | 58.66 | 209 | 133 |
100.00%
|
100.00%
|